Methods for judging the possibility of the onset of bovine leukemia and the resistance thereto

ABSTRACT

A method for judging a possibility of the onset of bovine leukemia caused by bovine leukemia virus BLV, wherein a bovine individual, in which an amino acid sequence defined by the amino acid numbers 75 to 78 of the β1 domain of the bovine MHC Class II DRβ chain is Val-Asp-Thr-Tyr, is judged to have a possibility of the onset of the leukemia: and a method for judging a resistance to the onset of bovine leukemia caused by the bovine leukemia virus BLV, wherein a bovine individual, in which an amino acid defined by the amino acid number 78 of the β1 domain of the bovine MHC Class II DRβ chain is Val, is judged to have a resistance to the onset of the leukemia.

This application is a 371 of PCT/JP97/02485 filed Jul. 17, 1997.

TECHNICAL FIELD

The present invention relates to a method for judging a possibility ofthe onset of bovine leukemia caused by bovine leukemia virus BLV and aresistance to the onset of the leukemia.

BACKGROUND ART

The major histocompatibility antigens (MHC antigens) are moleculesinvolved in self-nonself differentiation in the defense mechanism of theliving body against infection. They are classified into Class I moleculecomposed of α chain and β2M, and class II molecule composed of α chainand β chain. A groove for trapping an antigen peptide is present on theα1 and α2 domains, and also on the α1 and β1 domains. They are featuredto have the T cell receptor recognize only a fragmented peptide trappedin the groove, thereby achieve cell death (cellular immunity) by CD8+cells which have recognized the class I antigens, as well as inducemainly antibody production (humoral immunity) by CD4+ cells which haverecognized the class 11 II antigens.

The MHC genes constitute a gene group most full of polymorphism, and thelocations of pockets. shapes. sizes and properties of the peptidetrapping grooves are different among haplotypes. It is considered thatassociation conditicions of the trapped fragment peptides may varydepending on these differences, whict decide immune response and diseasesensitivity of each individual. The correlation between the MHChaplotypes and a resistance to a disease (disease insusceptibility) or apossibility of the onset of a disease (disease susceptibility) has beenreported, for example, as to human immune deficiency virus (HIV), humanT cell leukemia virus (HTLV) and malaria.

As for the bovine MHC (BoLA) class II genes. existence of DQA, DQB, DRA,DRB, DNA, DOB, DYA, and DYB genes has been estimated. DRB3, inter atia.which is one of the three genes (DRB1 to B3) identified on the DRBgenetic locus, has been known to encode a functional protein, andexistence of 73 alleles has been revealed so far. However, there isalmost no report about correlation between bovine infectious diseasesand the bovine MHC (BoLA) haplotypes.

In particular, as to the bovine leukemia virus (BLV), which has the genePX that regulates virus proliferation in the same manner as the humanimmunodeficiency virus (HIV) and is a retrovirus most related to HTLV-I,a research group in the United States has reported its relationship withthe bovine MHC (BoLA) haplotypes mainly focusing disease resistance;however, its relationship with possibility of onset of the leukemia hasnot been reported. The ratio of cattle infected by this virus (infectionrate in Japan) is 10-20%, and 1-2% of the infected cattle developsextremely malignant endemic bovine leukemia to die after a long latentperiod of 10-15 years. Therefore, economic loss of stockbreeders causedby the virus is very serious. If a possibility of the onset of a cattleafter BLV infection can be evaluated by the analysis of bovine MHC(BoLA) haplotypes, it becomes possible to preliminarily select diseaseresistant cattle for bleeding, and it is expected that extremely safecattle breeding can be continued.

Accordingly, an object of the present invention is to elucidate therelationship between the bovine leukemia virus (BLV) and the bovine MHC(BoLA) haplotypes, and to provide a method for convenient judgement of apossibility of the onset of leukemia of a cattle caused by the bovineleukemia virus (BLV) and a resistance to the onset of the leukemia bymeans of genetic engineering techniques. Another object of the presentinvention is to provide a primer set useful for the aforementionedmethod for judgement.

DISCLOSURE OF THE INVENTION

The inventors of the present invention previously analyzed the structureof DRB gene locus among the bovine MHC (BoLA) class II genes, andreported the structures of DRB3 gene (BoLA-DRB3) and the gene productthereof (Biochem. Biophys. Res. Commun., 209, pp.981-988, 1995). Theinventors fiirther studied the function of the gene and found that aportion is present, whose amino acid sequence is distinctly differentbetween a cattle developing the leukemia and a cattle not developing thedisease, in the gene product from the second exon (β1 domain) ofBoLA-DRB3 showing particularly noticeable polymorphism. They also foundthat the amino acid substitutions directly correlated with diseasesusceptibility to BLV and disease resistance. The present invention wasachieved on the basis of these findings.

The present invention thus provides a method for judging a possibilityof the onset of bovine leukemia caused by bovine leukemia virus BLV,wherein a bovine individual, in which an amino acid sequence defined bythe amino acid number from 75 to 78 of the β1 domain of the bovine MHCClass II DRβ chain is Val-Asp-Thr-Tyr (SEQ ID NO: 9), is judged to havea possibility of the onset of the leukemia. As preferred embodiments ofthe method of the present invention, there are provided theaforementioned method which is applied to a cattle infected by thebovine leukemia virus BLV; and the aforementioned method wherein abovine individual, in which an amino acid sequence defined by the aminoacid numbers 75-78 of the β1 domain of the bovine MHC Class II DRβ chainis Val-Asp-Thr-Tyr (SEQ ID NO: 9) in both of the alleles, is judged tohave a risk of the onset.

According to another embodiment of the method of the present invention,there is provided a method for judging a possibility of the onset ofbovine leukemia caused by the bovine leukemia virus BLV, which comprisesthe steps of:

(1) amplifying genomic DNA isolated from a bovine individual by thepolymerase chain reaction (PCR) to prepare a PCR product containing aDNA coding for a part or full length of the β1 domain of the bovine MHCClass II DRβ chain, and

(2) judging that the bovine individual, in which an amino acid sequencecorresponding to the amino acid number from 75 to 78 of the β1 domain ofthe bovine MHC Class II DRβ chain is Val-Asp-Thr (SEQ ID NO: 9) in theamino acid sequence encoded by the DNA contained in the PCR product, hasa possibility of the onset of the leukemia. A preferred embodiment ofthe aforementioned method comprises a step of digesting the PCR productby using PstI.

According to another aspect of the present invention, there is provideda method for judging a resistance to the onset of bovine leukemia causedby the bovine leukemia virus BLV, wherein a bovine individual, in whichan amino acid defined by the amino acid number 78 of the β1 domain ofthe bovine MHC Class II DRβ chain is Val, is judged to have resistant tothe onset of the leukemia. As preferred embodiments of the method of thepresent invention, there are provided the aforementioned method which isapplied to a cattle infected by the bovine leukemia virus BLV; theaforementioned method wherein the bovine individual, in which the aminoacid specified by the amino acid number 78 of the β1 domain of thebovine MHC Class II DRβ chain is Val in at least one of the alleles, isjudged to have a resistance to the onset; and the aforementioned methodwherein the bovine individual, in which the amino acid specified by theamino acid number 78 of the β1 domain of the bovine MHC Class II DRβchain is Val in both of the alleles, is judged to have a high resistanceto the onset.

According to another embodiment of the method of the present invention,there is provided a method for judging a resistance to the onset ofbovine leukemia caused by the bovine leukemia virus BLV, which comprisesthe steps of:

(1) amplifying genome DNA isolated from a bovine individual by thepolymerase chain reaction (PCR) to prepare a PCR product containing aDNA coding for a part or full length of the β1 domain of the bovine MHCClass II DRβ chain, and

(2) judging that the bovine individual, in which an amino acidcorresponding to amino acid number 78 of the β1 domain of the bovine MHCClass II DRβ chain is Val in the amino acid sequence encoded by the DNAcontained in the PCR product, has a resistance to the onset of theleukemia. A preferred embodiment of the aforementioned method comprisesa step of digesting the PCR product by using PstI.

According to preferred embodiments of these inventions, there areprovided each of the primer sets set out below, and the aforementionedmethods wherein said primer set is used. preferably in those applied tocattle infected by the bovine leukemia virus BLV. Tle present inventionfurther provides the following primer sets (1) to (3) each consisting ofA primer and B primer, which are used for judging a possibility of theonset of bovine leukemia caused by the bovine leukemia virus BLV:

Primer set (1)

A primer: 5'-TGTAAAACGACGGCCAGTCTCTCTCTGCAGCACATTTCCT-3'(SEQ ID NO: 1)

B primer: 5'-CAGGAAACAGCTATGACCCGCCGCTGCACAGTGAAACTC-3'(SEQ ID NO: 2)

Primer set (2)

A primer: 5'-GGAATTCCTCTCTCTGCAGCACATTTCCT-3'(SEQ ID NO: 3)

B primer: 5'-AAGTCGACCGCTGCACAGTGAAACTC-3'(SEQ ID NO: 4)

Primer set (3)

A primer: a primer which is selected from the group consisting of

5'-GAGTGTCATTTCTTCAACGGGAC-3'(SEQ ID NO: 5)

5'-GGAGAAGAGTTCGTGCGCTTCGA-3'(SEQ ID NO: 6), and

5'-GGAATTCCTCTCTCTGCAGCACATTTCCT-3'(SEQ ID NO: 7)

B primer: 5'-AAGTCGACCGCTGCACAGTGAAACTC-3'(SEQ ID NO: 8).

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 depicts the structure of the bovine MHC Class II DRβ chain. Inthe figure, (A) shows the structure of bovine MHC Class II DRβ chainmRNA, and (B) shows the full length cDNA coding for the bovine MHC ClassII DRβ chain and the amino acid sequence of the gene product. The β1domain is a portion defined by the amino acid sequence of the amino acidnumber from 1 to 94.

FIGS. 2(A) to (C) show the results of comparison of amino acids of theβ1 domain of the bovine MHC Class II DRβ chain (amino acid sequencesdefined by the amino acid number from 9 to 86) derived from cattleinfected by the bovine leukemia virus BLV but not developing the disease((A): 7 cattle developing lymphocytosis, and (B) and (C): antibodypositive 24 healthy cattle not developing the disease). The numbers atthe left end are ID numbers of bovine individuals, and amino acidsindicated as one letter symbols in the figure.

FIGS. 3 (A) and (B) show the results of comparison of amino acids of theβ1 domain of the bovine MHC Class II DRβ chain (amino acid sequencesdefined by the amino acid number from 9 to 86) derived from cattledeveloping leukemia (24 cattle). The numbers at the left end are IDnumbers of bovine individuals. and amino acids are indicated as oneletter symbols in the figure.

BEST MODE FOR CARRYING OUT THE INVNTION

The method of the present invention is applied to bovine individuals,including cattle infected with the bovine leukemia virus BLV and cattlenot infected with the virus, in order to judge a possibility of theonset of the leukemia of the individuals. Another method of the presentinvention is applied to bovine individuals, including cattle infectedwith the bovine leukemia virus BLV and cattle not infected with thevirus, in order to judge a resistance to the onset of the leukemia ofthe individuals.

According to a preferred embodiment of the present invention, genomicDNA of a bovine individual is isolated, and a gene coding for a part orthe full length of the β1 domain of DRβ chain of the bovine MHC Class II(the second exon of DRB3 gene) is amplified by the PCR method, and thenthe resulting PCR product is subjected to a sequencing to deduce theamino acid sequence defined by the amino acid number from 75 to 78 ofthe β1 domain. When a bovine individual, in which the amino acidsequence (amino acid numbers 75 to 78) is Val-Asp-Thr-Tyr (SEQ ID NO: 9)(indicated as VDTY in the one letter symbols), is already with infectionby the bovine leukemia virus BLV, or when the individual will sufferfrom infection by the bovine leukemia virus BLV, the bovine individualhas a possibility of the onset of the leukemia. Whether or not a bovineindividual is infected by the bovine leukemia virus BLV can be readilyverified by a test using an antibody recognizing the bovine leukemiavirus BLV.

In order to carry out more accurate judgement, it is preferred tocompare the aforementioned amino acid sequences in the alleles(haplotypes). When the amino acid sequence (amino acid number 75 to 78)is Val-Asp-Thr-Tyr (SEQ ID NO: 9) in both of the alleles (i.e., VDTYhomozygote), the bovine individual has a high risk of the onset of theleukemia when the individual is already infected by the bovine leukemiavirus BLV, or will suffer from the infection by the virus. On the otherhand, when the amino acid sequences in the alleles are heterozygote ofVal-Asp-Thr-Tyr (SEQ ID NO: 9) (VDTY) and Val-Asp-Thr-Val (SEQ ID NO:112) (VDTV); heterozygote of Val-Asp-Thr-Tyr (VDTY) (SEQ ID NO: 9) andVal-Asp-Arg-Val (VDRV) (SEQ ID NO: 113); homozygote of Val-Asp-Thr-Val(VDTV)(SEQ ID NO: 112); homozygote of Val-Aisp-Arg-Val (VDRV)(SEQ ID NO:113); heterozygote of Val-Asp-Arg-Val(VDRV) (SEQ ID NO: 113) andVal-Asp-Thr-Val (VDTV) (SEQ ID NO: 112) or the like, the bovineindividual has a very low possibility of the onset of the leukemia evenif the bovine individual is already infected by the bovine leukemiavirus BLV. or will suffer from the infection by the virus.

Furthermore, from a viewpoint of a resistance to the onset of heleukemia, the amino acid defined by the amino acid number 78 of the β1domain may be deduced. When a bovine individual having Val (representedas V in the one letter symbol) as the amino acid (i.e., amino acidnumber 78) is already infected by the bovine leukemia virus BLV, or willsuffer from infection by the virus, the bovine individual is resistantto the onset of the leukemia. Also for the judgement of the resistance,it is preferred to compare the aforementioned amino acid in the alleles(haplotypes). When the amino acid, defined by the amino acid number 78of the β1 domain, is Val in at least one of the alleles, the individualhas a resistance to the onset of the leukemia, and when the above aminoacid is Val in both of the alleles, the individual has a high resistanceto the onset of the leukemia.

The amino acid sequence of the β1 domain of the bovine MHC Class II DRβchain was reported by Aida et al. (Aida, Y., et al., Biochem. Biophys.Res. Commun., 209, pp.981-988, 1995). The structure of mRNA of thebovine MHC Class II DRβ chain (A), and the full length cDNA and theamino acid sequence of the gene product (B) are shown in FIG. 1. In thefigure, the β1 domain is a portion defined by the amino acid sequence ofamino acid number from 1 to 94, and the nucleotide sequence and theamino acid sequence are shown where the peptide sequence of the aminoacid number from 75 to 78 is "Val-Asp-Thr-Tyr (VDTY)". (SEQ ID NO: 9).

Cattle to be judged by the method according to the present invention arenot particularly limited. The method may be applied to any sorts ofcattle including dairy cattle, dairy and beef cattle, beef cattle,working cattle, working and beef cattle and the like, so long as theymay be infected by the bovine leukemia virus BLV and have a possibilityof developing the leukemia owing to the infection. More specifically,examples include Japanese cattle such as Japanese Black and JapaneseShorthorn, or breeds such as Holstein, Jersey, Hereford, Aberdeen Angus,and Friesian. However, breeds are not limited to these examples.

As a sample for preparing genomic DNA from bovine individuals,peripheral blood. organ and the like can be utilized. For example. atissue section of the lymph node and other may be used as the organ. Asmethods for preparing genomic DNA from the sample mentioned above, anymethods available to those skilled in the art can be employed. Whenperipheral blood leucocytes or peripheral blood lymphocytes are used asa sample, for example, the method of Hughes et al. (Hughes, S. H., Cell,15, pp.1397-1410, 1978) may be applied. When an organ is used. forexample, a frozen tissue section may be sliced by using scissors, andthen treated by the sodium dodecylstilfate and phenol-chloroform method(Mcknight, G. S., Cell, 14, pp.403-413, 1978) to obtain genomic DNA. Thesimplified extraction of genomic DNA from cells may also be used, whosedetails are described in the examples.

As primers used for amplifying the resulting genomic DNA by the PCRmethod, any primers may be used so long as they can amplify a DNAcontaining a gene coding for a partial amino acid sequence of amino acidnumber from 75 to 78 of the β1 domain of the DRβ chain of the bovine MHCClass II or the full length of the β1 domain.

An example of a primer set most suitably used for the methods of thepresent invention includes primer set (1):

A primer: 5'-TGTAAAACGACGGCCAGTCTCTCTCTGCAGCACATTTCCT-3'(SEQ ID NO: 1);and

B primer: 5'-CAGGAAACAGCTATGACCCGCCGCTGCACAGTGAAACTC-3'(SEQ ID NO: 2)

which enables direct sequencing methods such as the cycle sequencing andthe Dynabeads DNA direct sequencing. As primer sets introduced with arestriction endonuclease cleavage site, primer set (2):

A primer: 5'-GGAATTCCTCTCTCTGCAGCACATTTCCT-3'(SEQ ID NO: 3); and

B primer: 5'-AAGTCGACCGCTGCACAGTGAAACTC-3(SEQ ID NO: 4),

or primer set (3):

A primer: a primer selected from the group consisting of:

5'-GAGTGTCATTTCTTCAACGGGAC-3'(SEQ ID NO: 5),

5'-GGAGAAGAGTTCGTGCGCTTCGA-3'(SEQ ID NO: 6), and

5'-GGAATTCCTCTCTCTGCAGCACATTTCCT-3'(SEQ ID NO: 7); and

B primer: 5'-AAGTCGACCGCTGCACAGTGAAACTC-3'(SEQ ID NO: 8) may beutilized. In particular, by digesting PCR alleles with PstI that areamplified by using the primer set (3), and then observing differences inthe resulting cleavage patterns, it can easily judge whether or not thebovine individual is resistant to the leukemia, or whether or not theindividial has a possibility of the onset of the leukemia. However,primers and primer sets which may be used for the methods of the presentinvention are not limited to the forgoing examples.

An amount of DNA used for the PCR method can be appropriately chosen.For example, the amount may be about 0.1-0.5 μg when peripheral bloodleucocytes or peripheral lymphocytes are used. As sequencing methodsapplied to the DNA amplified as descried above (the PCR product), anymethods available to those skilled in the art may be utilized. Forexample, the direct sequencing may preferably be used, whose specificexamples are described in the examples. Most of cattle areheterozygotes, and when alleles derived from father and mother cattlemay have different nucleotide sequences, the direct sequencing may failto determine which of the alleles corresponds to the target sequence. Inthat case, the PCR product amplified by using the above primer set (2)may be digested with restriction endonuclease EcoRI and Sal I, and thensubcloned into a vector to carry out the sequencing of only one of thealleles, and the results may be referred to for comparison to enable adefinite sequencing of the other allele. To obtain more precise geneticinformation, it is preferred that both of the alleles from the PCRproduct are subcloned and each of the nucleotide sequences isdetermined. The specific method and applicable primers are detailed inthe following examples.

EXAMPLES

The present invention will be explained more specifically by referringto examples. However, the scope of the present invention is not limitedto the examples set out below.

Example 1

Examination of a Possibility of the Onset of the Leukemia

Peripheral blood was collected as a sample from a bovine individual byusing a syringe containing an anticoagulant, and centrifuged underconditions of 4° C. and 3,000 rpm for 20 minutes to obtain a leucocytelayer. The separated leucocyte layer was washed with phosphate bufferedsaline (PBS) and centrifuged to obtain a pellet, which washed as asample of periipheral blood leucocyte. Peripheral blood lymphocytes werealso obtained by the method of Miyasaka et al. (Miyasaka, M. and Trnka,Z., Immunological Methods, Vol.3, pp.403-423, 1985, Academic Press, NY)from peripheral blood obtained in the same manner as described above,and a sample of peripheral lymphocyte was prepared by obtaining a pelletas described above. A BLV infected cell suspension was centrifuged underconditions of 4° C. and 1,100 rpm for 5 minutes to remove a culturemedium, and the cells were washed with PBS and centrifuged to obtain apellet as a sample. In addition, tissue sections were isolated from thelymph node and a tumor tissue of a cattle which developed BLV infectionlymphosarcoma, and rapidly frozen in liquid nitrogen withoutimmobilization, and then stored at -80° C. as samples of the tissuesections.

Each of the above sample cells were washed twice with PBS in a 1.5ml-microcentrifugal tube, and the precipitated cells were suspendedagain in PBS by using a vortex mixer. To 1×10⁶ cells, 200 μl of 1×PCRbuffer [10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2.5 mM MgCl₂, 0.5% Tween-20]and 1 μl of Proteinase K (20 mg/ml) were added, and the cells weresuspended again by a vortex mixer and incubated at 56° C. for 45-60minutes. The mixture was further treated at 95° C. for ten minutes, andcooled on ice for 5 minutes or more. About 5-10 μl of the reactionmixture was used for amplification by PCR.

The genome DNA was dissolved in 50 μl of 1×PCR buffer [10 mM Tris-HCl(pH 8.3), 50 mM KCl, 1.5 mM MgCl₂, 0.001% (w/v) gelatin] containing 200μM of each dNTP, 0.2-0.4 μM of primers, and 2.5 units of Taq polymerase(Gene Amp Kit; Perkin-Elmer Cetus), and then subjected to amplificationby 25 cycles, each cycle consisting of treatments at 94° C. for 1minute, at 61° C. for 1 minute, and at 72° C. for 1 minute, and thenfurther treated at 72° C. for 5 minutes. As the primers, the followingprimers were used:

A primer: 5'-TGTAAAACGACGGCCAGTCTCTCTCTGCAGCACATTTCCT-3'(SEQ ID NO: 1)

B primer: 5'-CAGGAAACAGCTATGACCCGCCGCTGCACAGTGAAACTC-3'(SEQ ID NO: 2)

which can specifically amplify the β1 domain of the bovine MHC Class IIDRβ chain (β1 domain of BoLA-DRβ: the second exon of DRB3 gene) by thePCR method. Specific biotinylation was introduced into the 5' end of theB primer. These primers can be suitably used for the cycle sequencing.

20 μl of DYNABEADS M-280 Streptoavidin (Dynal A. S, N-0212, Oslo,Norway) was washed with 100 μl of 2×binding-washing buffer (B&W buffer:10 mM Tris-HCl (pH 7.5). 1.0 mM EDTA. 2M NaCl, 0.1% Tween-20), and thebeads were suspended again in 80 μl of 2×B&W buffer. The above PCRproduct (50 μl ) was added to the bead suspension and gently mixed bypipetting, and then incubated at room temperature for 15 minutes withslow rotation using a wheel rotator. The tube containing the immobilizedPCR product was put on a magnet (Dynal MPC) and the supernatant wasremoved by using a pipet, and then 100 μl of 2×B&W buffer was added towash the beads. The supernatant was removed again by using a magnet, andthe residue was suspended in 50 μl of 0.1 M NaOH prepared just beforeuse.

The beads immobilizing the biotinylated chains were gathered on the tubewall by using a magnet and the supernatant was removed, and then thebeads were washed once with 50 μl of 0.1 M NaOH and three times with 100μl of 1×B&W buffer, and once with 50 μl of TE buffer. In everyoperation, the beads were resuspended with smooth strokes. After washingwith 100 μl of distilled water, the supernatant was removed, anddistilled water was added to the residue to adjust the volume for theuse in a sequencing. The sequencing was performed by using BcaBESTDideoxy Sequencing Kit (Takara Biomedicals) and according to theconditions described in the attached instructions. The following primerswere used as sequencing primers.

Forward primer: 5'-TGTAAAACGACGGCCAGT-3'(SEQ ID NO: 114)

Reverse primer: 5'-CAGGAAACAGCTATGACC-3'(SEQ ID NO: 115)

The results are shown in FIGS. 2 and 3 (in the figures, amino acids ofnumber 9 to 86 of the β1 domains of the bovine MHC Class II DRβ areshown, and the numbers at the left end are ID numbers of bovineindividuals). By comparing the amino acids of the β1 domain of bovineMHC Class II DRβ derived from cattle infected by the bovine leukemiavirus but not developing the leukemia [7 cattle with lymphocytosis(pre-cancer state), and 24 cattle not developing the leukemia (antibodypositive healthy cattle not developing the disease), top and bottom ofFIG. 2, respectively], and cattle already developing the leukemia (24cattle, FIG. 3), a markedly characteristic result was obtained th at thecattle with the developed leukemia had Val-Asp-Thr-Tyr (VDTY) (SEQ IDNO: 9) motif as the sequence of amino acid number from 75 to 78 in bothof the alleles. The portion of the amino acids from 75 to 78 is locatedon an α-helix of the β1 domain, and may have a function as a T cellrecognition site. Furthermore, as a result of an analysis using acomputer, it was revealed that this motif exists only in pol protein inthe bovine leukemia virus BLV.

The above results are summarized in Table 1. The representation of thegenotype such as VDTY/VDTY in the table indicates amino acid sequencesof the both allele (amino acid number 75 to 78 of the μl domain of thebovine MHC Class II DRβ chain) described as the one letter symbols. Theinfection status of the BLV infected cattle were classified according tothe criteria of Levy et al. (Levy, D., et al., Int. J. Cancer. 19,pp.822-827, 1977) and Aida et al. (Aida, Y., et al., Cancer Res., 52,pp.6463-6470, 1992).

                  TABLE 1                                                         ______________________________________                                        BLV infection status (positive rate)                                                     Development                                                                   of                                                                 Genotype   leukemia     Lymphocytosis                                                                            Healthy                                    ______________________________________                                        VDTY/VDTY  19/24        5/7        4/24                                       VDTY/VDTV  2/24         2/7        2/24                                       VDTY/VDRV  2/24         0/7        14/24                                      VDRV/VDRV  0/24         0/7        1/24                                       VDTV/VDTV  1/24         0/7        0/24                                       VDTV/VDRV  0/24         0/7        3/24                                       ______________________________________                                    

Example 2

Study on Resistance to the Onset of Leukemia

It the same manner as in Example 1, kinds of the amino acid at number 78of the β1 domains of bovine MHC Class II DRβ was determined for cattledeveloping the leukemia (24 cattle), cattle not developing the leukemia(cattle with lymphocytosis and healthy cattle, 31 individuals in total),and the results are shown in Table 2. The kind of the amino acids atnumbers 71 and 74 were also determined (in the table, amino acids areindicated as one letter symbols, Y: Tyr; V: Val; R: Arg; E: Glu; K: Lys;and N: Asn). As a result, it was revealed that individuals where the78th amino acid was heterozygote of valine and tyrosine, and individualswhere the 78th amino acid was homozygote of valine were resistant to theonset of the leukemia, and in particular, the individuals where the 78thamino acid was homozygote of valine where lighly resistant to the onsetof the leukemia. Furthermore, because all of the 74th amino acids ofcattle not developing the leukemia were Gln or Asn, and the 71st aminoacid residues were Lys or Arg, it was suggested that individuals havingthe allele where the 71st amino acid is lysine or arginine, the 74thamino acid is glutamic acid or asparagine, and the 78th amino acid isvaline have high resistant to the onset of the leukemia.

                  TABLE 2                                                         ______________________________________                                                                       Positive                                       Genotype   BLV infection status                                                                              rate                                           ______________________________________                                        Y.sup.78 /Y.sup.78                                                                       Cattle developing leukemia                                                                        19/24                                          V.sup.78 /Y.sup.78                                                                       Cattle developing leukemia                                                                        4/24                                                      (R.sup.71 -E.sup.74 -V.sup.78 /Y.sup.78 :                                                         3/24)                                                     (K.sup.71 -E.sup.74 -V.sup.78 /Y.sup.78 :                                                         1/24)                                                     (K.sup.71 -N.sup.74 -V.sup.78 /Y.sup.78 :                                                         0/24)                                          V.sup.78 /V.sup.78                                                                       Cattle developing leukemia                                                                        1/24                                                      (R.sup.71 -E.sup.74 -V.sup.78 /R.sup.71 -E.sup.74 -V.sup.78                                       1/24)                                                     (K.sup.71 -E.sup.74 -V.sup.78 /K.sup.71 -E.sup.74 -V.sup.78                                       0/24)                                                     (K.sup.71 -N.sup.74 -V.sup.78 /K.sup.71 -N.sup.74 -V.sup.78                                       0/24)                                          Y.sup.78 /Y.sup.78                                                                       Cattle not developing leukemia                                                                    9/31                                           V.sup.78 /Y.sup.78                                                                       Cattle not developing leukemia                                                                    18/31                                                     (R.sup.71 -E.sup.74 -V.sup.78 /Y.sup.78 :                                                         11/31)                                                    (K.sup.71 -E.sup.74 -V.sup.78 /Y.sup.78 :                                                         4/31)                                                     (K.sup.71 -N.sup.74 -V.sup.78 /Y.sup.78 :                                                         3/31)                                          V.sup.78 /V.sup.78                                                                       Cattle not developing leukemia                                                                    4/31                                                      (R.sup.71 -E.sup.74 -V.sup.78 /R.sup.71 -E.sup.74 -V.sup.78                                       3/31)                                                     (K.sup.71 -E.sup.74 -V.sup.78 /K.sup.71 -E.sup.74 -V.sup.78                                       1/31)                                                     (K.sup.71 -N.sup.74 -V.sup.78 /K.sup.71 -N.sup.74 -V.sup.78                                       0/31)                                          ______________________________________                                    

Example 3

Method for Quick Judgement of Possibility and Resistance to the Onset

As described above, individuals having the gene coding for Val as theamino acid at number 78 of the β1 domain of the bovine MHC Class II DRβchain are resistant to the leukemia caused by the bovine leukemia virus,whereas individuals where the 78th amino acid is Tyr in both of thealleles have a possibility of the onset of the leukemia. Therefore.whether or not an bovine individual is leukemia resistant, or whether ornot an individual has a possibility of the onset of the leukemia iseasily judged by utilizing restriction endonuclease PstI cleavage sitewhich is present in a gene where the 78th allele is Val but absent in agene where the 78th allele is Tyr, i.e., by digesting PCR amplifiedalleles and differentiating the cleavage pattern.

The following primers were used as PCR primers.

A primer

DRB40: 5'-GAGTGTCATTTCTTCAACGGGAC-3'(SEQ ID NO: 5)

DRB100: 5'-GGAGAAGAGTTCGTGCGCTTCGA-3'(SEQ ID NO: 6)

ERB3: 5'-GGAATTCCTCTCTCTGCAGCACATTTCCT-3'(SEQ ID NO: 7)

B primer

SRB3: 5'-AAGTCGACCGCTGCACAGTGAAACTC-3'(SEQ ID NO: 8).

The conditions of the PCR were similar to the conditions of Example 1.Specifically, amplification was performed by 35 cycles, each cycleconsisting of the following steps depending on the combination of theprimers, followed by a treatment at 72° C. for 10 minutes. The genomicDNA was used in an amount of 100 ng for 100 μl of the PCR system.

DRB40/SRB3: 94° C. for 1 minute, 63° C. for 2 minutes, 72° C. for 2minutes

DRB100/SRB3: 94° C. for 1 minute, 66° C. for 2 minutes, 72° C. for 2minutes

ERB3/SRB3: 94° C. for 1 minute, 61° C. for 2 minutes, 72° C. for 2minutes

The PCR product was subjected to 2% agarose gel electrophoresis, andthen cleaved by using restriction endonuclease PstI (1.2 μl of10×restriction endonuclease buffer, 6-7 μl of DNA after amplification, 2units of restriction endonuclease PstI, and H₂ O in the total volume of12 μl). After completion of the reaction with the restrictionendonuclease, each specimen was examined by 3% agarose gelelectrophoresis for judgement. The results are shown in Table 3.

                  TABLE 3                                                         ______________________________________                                               PCR   Size (bp) of PstI fragment                                                product Allele                                                       Primer   (bp)    for Y   Allele for V                                                                          Y/Y   Y/V                                    ______________________________________                                        DRB40/SRB3                                                                             247     199, 48 247     199, 48                                                                             247, 199, 48                           DRB100/SRB3                                                                            187     139, 48 187     139, 48                                                                             139, 187, 48                           ERB3/SRB3*                                                                             292     226, 48 274     226, 48                                                                             226, 274, 48                           ______________________________________                                         *PstI cleavage site is present in the ERB3 primer, and hence a 18 bp          fragment was contained in each reaction mixture.                         

INDUSTRIAL APPLICABILITY

A possibility of the onset of the leukemia caused by the bovine leukemiavirus (BLV) and a resistance thereto of a bovine individual can besurely estimated by the methods of the present invention. Therefore, theinvention enables safe cattle breeding and achieves prevention ofeconomic loss of stockbreeders.

    __________________________________________________________________________    #             SEQUENCE LISTING                                                - <160> NUMBER OF SEQ ID NOS: 115                                             - <210> SEQ ID NO 1                                                           <211> LENGTH: 40                                                              <212> TYPE: DNA                                                               <213> ORGANISM: Artificial Sequence                                           <220> FEATURE:                                                                #Sequence: PRIMER FORON: Description of Artificial                                  JUDGING POSSIBILITY OF ONSET OF B - #OVINE LEUKEMIA                     - <400> SEQUENCE: 1                                                           #    40            gtct ctctctgcag cacatttcct                                 - <210> SEQ ID NO 2                                                           <211> LENGTH: 39                                                              <212> TYPE: DNA                                                               <213> ORGANISM: Artificial Sequence                                           <220> FEATURE:                                                                #Sequence: PRIMER FORON: Description of Artificial                                  JUDGING POSSIBILITY OF ONSET OF B - #OVINE LEUKEMIA                     - <400> SEQUENCE: 2                                                           #    39            cccg ccgctgcaca gtgaaactc                                  - <210> SEQ ID NO 3                                                           <211> LENGTH: 29                                                              <212> TYPE: DNA                                                               <213> ORGANISM: Artificial Sequence                                           <220> FEATURE:                                                                #Sequence: PRIMER FORON: Description of Artificial                                  JUDGING POSSIBILITY OF ONSET OF B - #OVINE LEUKEMIA                     - <400> SEQUENCE: 3                                                           #            29    cagc acatttcct                                             - <210> SEQ ID NO 4                                                           <211> LENGTH: 26                                                              <212> TYPE: DNA                                                               <213> ORGANISM: Artificial Sequence                                           <220> FEATURE:                                                                #Sequence: PRIMER FORON: Description of Artificial                                  JUDGING POSSIBILITY OF ONSET OF B - #OVINE LEUKEMIA                     - <400> SEQUENCE: 4                                                           #              26  agtg aaactc                                                - <210> SEQ ID NO 5                                                           <211> LENGTH: 23                                                              <212> TYPE: DNA                                                               <213> ORGANISM: Artificial Sequence                                           <220> FEATURE:                                                                #Sequence: PRIMER FORON: Description of Artificial                                  JUDGING POSSIBILITY OF ONSET OF B - #OVINE LEUKEMIA                     - <400> SEQUENCE: 5                                                           #                23acgg gac                                                   - <210> SEQ ID NO 6                                                           <211> LENGTH: 23                                                              <212> TYPE: DNA                                                               <213> ORGANISM: Artificial Sequence                                           <220> FEATURE:                                                                #Sequence: PRIMER FORON: Description of Artificial                                  JUDGING POSSIBILITY OF ONSET OF B - #OVINE LEUKEMIA                     - <400> SEQUENCE: 6                                                           #                23gctt cga                                                   - <210> SEQ ID NO 7                                                           <211> LENGTH: 29                                                              <212> TYPE: DNA                                                               <213> ORGANISM: Artificial Sequence                                           <220> FEATURE:                                                                #Sequence: PRIMER FORON: Description of Artificial                                  JUDGING POSSIBILITY OF ONSET OF B - #OVINE LEUKEMIA                     - <400> SEQUENCE: 7                                                           #            29    cagc acatttcct                                             - <210> SEQ ID NO 8                                                           <211> LENGTH: 26                                                              <212> TYPE: DNA                                                               <213> ORGANISM: Artificial Sequence                                           <220> FEATURE:                                                                #Sequence: PRIMER FORON: Description of Artificial                                  JUDGING POSSIBILITY OF ONSET OF B - #OVINE LEUKEMIA                     - <400> SEQUENCE: 8                                                           #              26  agtg aaactc                                                - <210> SEQ ID NO 9                                                           <211> LENGTH: 4                                                               <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 9                                                           - Val Asp Thr Tyr                                                               1                                                                           - <210> SEQ ID NO 10                                                          <211> LENGTH: 1141                                                            <212> TYPE: DNA                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 10                                                          - ctctgctgtt ctccggcatg gtgtgcctgt atttctctgg aggctcctgg at - #ggcagctc         60                                                                          - tgatagtgat gctgatggtg ctgtgccctc ccctggcctg ggccagggag at - #ccaaccac        120                                                                          - atttcctgga gtataccaag aaagagtgtc atttcttcaa cgggaccgag cg - #ggtgcggt        180                                                                          - tcctggacag atacttccat aatggagaag agttcgtgcg cttcgatagc ga - #ctggggcg        240                                                                          - agtaccgggc ggtgaccgag ctagggcggc cggacgccaa gtactggaac ag - #ccagaagg        300                                                                          - acttcctgga ggagaagcgg gccgcggtgg acacgtactg cagacacaac ta - #cggggtcg        360                                                                          - gtgagagttt cactgtgcag cggcgagtgg aacctatagt gactgtgtat cc - #tgcaaaga        420                                                                          - cccagcccct gcagcaccac aacctcctgg tctgctctgt gaacggtttc ta - #cccaggca        480                                                                          - acattgaagt caggtggttc cggaatggcc atgaagagga ggctggggtg at - #ctccacag        540                                                                          - gcctgatcca gaatggagac tggaccttcc agaccatggt gatgcttgaa ac - #agttcctc        600                                                                          - agagtggaga ggtctacacc tgccaagtgg agcaccccag ccagacaagc cc - #tatcacag        660                                                                          - tagaatggag ggcacggtct gactctgctc agagcaagat gatgagtgga gt - #cgggggct        720                                                                          - tcgttctggg tctgttcttc cttgccgtgg ggctcttcat ctacttcagg aa - #tcagaaag        780                                                                          - gacgccctac acttcagcca acagggctcc tgagctgaag tgaagatggt ca - #cactcaag        840                                                                          - gaagaacctt ctgtcccagc ttcttcacag catggaaagg tttcctgctt ag - #tgctaact        900                                                                          - cttccacaat gaagtacttt ctcaggatct catttgctcc tggctcagtg ac - #cccttaaa        960                                                                          - aactgtcctc gatggttttc tcagtcacct ccaccctgct gccctcagcc tt - #tgacctgg       1020                                                                          - aagttctcaa tattgattcc agtaccttat gttctttctt ccttggttcc ct - #ttcttttc       1080                                                                          - aacttctgtt tcctgtgcat ctgagctcat ctgttcattt tactttataa tg - #tgttctct       1140                                                                          #             1141                                                            - <210> SEQ ID NO 11                                                          <211> LENGTH: 266                                                             <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 11                                                          - Met Val Cys Leu Tyr Phe Ser Gly Gly Ser Tr - #p Met Ala Ala Leu Ile         #                 15                                                          - Val Met Leu Met Val Leu Cys Pro Pro Leu Al - #a Trp Ala Arg Glu Ile         #             30                                                              - Gln Pro His Phe Leu Glu Tyr Thr Lys Lys Gl - #u Cys His Phe Phe Asn         #         45                                                                  - Gly Thr Glu Arg Val Arg Phe Leu Asp Arg Ty - #r Phe His Asn Gly Glu         #     60                                                                      - Glu Phe Val Arg Phe Asp Ser Asp Trp Gly Gl - #u Tyr Arg Ala Val Thr         # 80                                                                          - Glu Leu Gly Arg Pro Asp Ala Lys Tyr Trp As - #n Ser Gln Lys Asp Phe         #                 95                                                          - Leu Glu Glu Lys Arg Ala Ala Val Asp Thr Ty - #r Cys Arg His Asn Tyr         #           110                                                               - Gly Val Gly Glu Ser Phe Thr Val Gln Arg Ar - #g Val Glu Pro Ile Val         #       125                                                                   - Thr Val Tyr Pro Ala Lys Thr Gln Pro Leu Gl - #n His His Asn Leu Leu         #   140                                                                       - Val Cys Glu Val Asn Gly Phe Tyr Pro Gly As - #n Ile Glu Val Arg Trp         145                 1 - #50                 1 - #55                 1 -       #60                                                                           - Phe Arg Asn Gly His Glu Glu Glu Ala Gly Va - #l Ile Ser Thr Gly Leu         #               175                                                           - Ile Gln Asn Gly Asp Trp Thr Phe Gln Thr Me - #t Val Met Leu Glu Thr         #           190                                                               - Val Pro Gln Ser Gly Glu Val Tyr Thr Cys Gl - #n Val Glu His Pro Ser         #       205                                                                   - Gln Thr Ser Pro Ile Thr Val Glu Trp Arg Al - #a Arg Ser Asp Ser Ala         #   220                                                                       - Gln Ser Lys Met Met Ser Gly Val Gly Gly Ph - #e Val Leu Gly Leu Phe         225                 2 - #30                 2 - #35                 2 -       #40                                                                           - Phe Leu Ala Val Gly Leu Phe Ile Tyr Phe Ar - #g Asn Gln Lys Gly Arg         #               255                                                           - Pro Thr Leu Gln Pro Thr Gly Leu Leu Ser                                     #           265                                                               - <210> SEQ ID NO 12                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 12                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Ly - #s Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Glu Lys Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 13                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 13                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asn Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 14                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 14                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Ser Arg Arg Thr Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Phe Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 15                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 15                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Cy - #s Lys Arg Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Ser Tyr         #             30                                                              - Asn Gly Lys Glu Arg Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ser Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Gln Arg Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 16                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 16                                                          - Arg Glu Ile Gln Pro His Phe Leu Gln Tyr Hi - #s Lys Gly Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg His Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Asp Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ser Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Arg Arg Ala Glu Va - #l Asp Thr Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 17                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 17                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Asp Arg Val Arg Phe Le - #u Asn Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 18                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 18                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Tyr Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Arg Val Ala Gl - #u Gln Leu Asn Gly Gln         #     60                                                                      - Lys Asp Thr Leu Glu Arg Glu Arg Ala Tyr Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 19                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 19                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Ser Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Ala Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Ser Arg Arg Thr Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Ile Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 20                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 20                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Cy - #s Lys Arg Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Cys Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Arg Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Arg Val Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Glu Lys Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 21                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 21                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asn Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Gly Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 22                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 22                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Gln Arg Val Ala Gl - #u Tyr Cys Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 23                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 23                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Thr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Gln Asp Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - Tyr Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 24                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 24                                                          - Arg Glu Ile Gln Pro His Phe Leu Gln Tyr Hi - #s Lys Gly Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg His Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Asp Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ser Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Arg Arg Ala Glu Va - #l Asp Thr Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 25                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 25                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Glu Arg Ser Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Glu Arg Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 26                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 26                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Glu Arg Ser Phe Tyr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Lys Arg Ala Asn Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 27                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 27                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asn Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 28                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 28                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Glu Arg Ser Phe Tyr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Tyr Leu Glu Glu Arg Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 29                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 29                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Glu Arg Ser Phe Tyr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Lys Arg Ala Asn Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 30                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 30                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asn Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Ile Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 31                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 31                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Cy - #s Lys Arg Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Cys Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Arg Val Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Glu Arg Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 32                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 32                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asn Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 33                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 33                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Cy - #s Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Glu Arg Ser Phe Tyr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Glu Arg Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 34                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 34                                                          - Arg Glu Ile Gln Pro His Phe Leu Gln Tyr Hi - #s Lys Gly Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg His Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Asp Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ser Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Arg Arg Ala Glu Va - #l Asp Thr Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 35                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 35                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Le - #u Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Glu Arg Ser Phe Tyr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Ly - #s Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Leu Leu Glu Arg Lys Arg Ala Asn Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 36                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 36                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Cy - #s Lys Arg Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Cys Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Arg Val Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Glu Arg Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 37                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 37                                                          - Arg Glu Ile Gln Pro His Phe Leu Gln Tyr Hi - #s Lys Gly Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg His Phe Tyr         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Asp Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ala Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Gln Lys Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 38                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 38                                                          - Arg Glu Ile Gln Pro His Phe Leu Gln Tyr Hi - #s Lys Gly Glu Cys His         #                 15                                                          - Phe Leu Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg His Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Asp Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ser Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Arg Arg Ala Glu Va - #l Asp Thr Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 39                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 39                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Thr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Gln Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Glu Lys Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Gly Met Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 40                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 40                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Glu Arg Ser Phe Tyr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Lys Arg Ala Asn Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 41                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 41                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asn Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 42                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 42                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Ser Phe Tyr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Glu Arg Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 43                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 43                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Glu Arg Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 44                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 44                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asn Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 45                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 45                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Ser Arg Arg Thr Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Phe Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 46                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 46                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Ser Phe Tyr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Ile Leu Glu Glu Arg Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 47                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 47                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Tyr Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Arg Val Ala Gl - #u Gln Leu Asn Gly Gln         #     60                                                                      - Lys Asp Thr Leu Glu Arg Glu Arg Ala Tyr Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 48                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 48                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Ser Arg Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Phe Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 49                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 49                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asn Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 50                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 50                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Al - #a Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Leu Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ser Ala Va - #l Pro Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Gly Glu Arg Ala Ser Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 51                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 51                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Cy - #s Lys Arg Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Glu Arg Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 52                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 52                                                          - Arg Glu Ile Gln Pro His Phe Leu Gln Tyr Hi - #s Lys Gly Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg His Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Asp Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ser Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Arg Arg Ala Glu Va - #l Asp Thr Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 53                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 53                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Glu Arg Ser Phe Tyr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Glu Arg Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 54                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 54                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Tyr Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Arg Val Ala Gl - #u Gln Leu Asn Gly Gln         #     60                                                                      - Lys Asp Thr Leu Glu Arg Glu Arg Ala Tyr Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 55                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 55                                                          - Arg Glu Ile Gln Pro His Phe Leu Gln Tyr Hi - #s Lys Gly Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg His Phe Tyr         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Asp Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ala Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Gln Lys Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Gly Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 56                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 56                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asn Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 57                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 57                                                          - Arg Glu Ile Gln Pro His Phe Leu Gln Tyr Hi - #s Lys Gly Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg His Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Asp Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ser Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Arg Arg Ala Glu Va - #l Asp Thr Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 58                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 58                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 59                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 59                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Thr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Gln Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Glu Lys Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Gly Met Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 60                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 60                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asn Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Ala Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 61                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 61                                                          - Arg Glu Ile Gln Pro His Phe Leu Gln Tyr Hi - #s Lys Gly Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg His Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Asp Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ser Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Arg Arg Ala Glu Va - #l Asp Thr Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 62                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 62                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Leu Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Gly Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 63                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 63                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Thr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Gln Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Glu Lys Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Gly Met Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 64                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 64                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asn Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Gly Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 65                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 65                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Gln Arg Val Ala Gl - #u Tyr Cys Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 66                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 66                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Glu Arg Ser Phe Tyr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Asp Leu Glu Glu Arg Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 67                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 67                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 68                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 68                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Cy - #s Lys Arg Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ala Ala Ly - #s Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Glu Lys Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 69                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 69                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Glu Arg Ser Phe Tyr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Arg Val Ala Gl - #u Gln Leu Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Gln Lys Arg Ala Asn Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 70                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 70                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Ly - #s Tyr Arg Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Glu Lys Arg Ala Gln Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 71                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 71                                                          - Arg Glu Ile Gln Pro His Phe Leu Gln Tyr Hi - #s Lys Gly Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg His Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Asp Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ser Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Arg Arg Ala Glu Va - #l Asp Thr Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 72                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 72                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asn Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 73                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 73                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Gln Arg Val Ala Gl - #u Tyr Cys Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 74                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 74                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Gln Arg Val Ala Gl - #u Tyr Ser Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 75                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 75                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Gln Arg Val Ala Gl - #u Tyr Cys Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 76                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 76                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Gln Arg Val Ala Gl - #u Tyr Cys Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 77                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 77                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Ser Arg Arg Thr Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Phe Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 78                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 78                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Glu Arg Ser Phe Tyr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Glu Arg Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 79                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 79                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Tyr Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Arg Val Ala Gl - #u Gln Leu Asn Gly Gln         #     60                                                                      - Lys Asp Thr Leu Glu Arg Glu Arg Ala Tyr Va - #l Asp Tyr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 80                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 80                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Tyr Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Arg Val Ala Gl - #u Gln Leu Asn Gly Gln         #     60                                                                      - Lys Asp Thr Leu Glu Arg Glu Arg Ala Tyr Va - #l Asp Tyr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 81                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 81                                                          - Arg Glu Ile Gln Pro His Phe Leu Gln Tyr Hi - #s Lys Gly Glu Cys His         #                 15                                                          - Phe Leu Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg His Phe His         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Asp Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ser Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Arg Arg Ala Glu Va - #l Asp Thr Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 82                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 82                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Hi - #s Lys Gly Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg Tyr Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Asp Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ser Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Arg Arg Ala Glu Va - #l Asp Thr Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 83                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 83                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Hi - #s Lys Ser Glu Cys Arg         #                 15                                                          - Phe Phe His Gly Thr Glu Arg Val Arg Tyr Le - #u Asp Arg Tyr Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Asn As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Ala Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Lys Arg Ala Glu Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Ile Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 84                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 84                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Ser Arg Arg Thr Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Phe Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 85                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 85                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Ala Glu Leu Gly Arg Pro Glu Ala Ly - #s Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Ile Leu Glu Arg Lys Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Ile Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 86                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 86                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Ty - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Thr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Leu Thr Glu Leu Gly Arg Gln Asp Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - Tyr Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 87                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 87                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Arg Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp His Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Gly Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 88                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 88                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Ty - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Gly Glu Thr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Leu Thr Glu Leu Gly Arg Gln Asp Ala Gl - #y Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - Tyr Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 89                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 89                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Leu Le - #u Glu Arg Tyr Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Ala Glu Leu Gly Arg Pro Asp Ala Ly - #s Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Ser Arg Arg Thr Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 90                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 90                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Ser Arg Arg Thr Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Phe Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 91                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 91                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Ser Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Ala Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Ser Arg Arg Thr Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Ile Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 92                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 92                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Tyr Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Arg Val Ala Gl - #u Gln Leu Asn Gly Gln         #     60                                                                      - Lys Asp Thr Leu Glu Arg Glu Arg Ala Tyr Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 93                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 93                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Glu Ala Ly - #s Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Lys Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Ile Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 94                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 94                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Ty - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Thr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Leu Thr Glu Leu Gly Arg Gln Asp Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 95                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 95                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Ty - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Thr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Leu Thr Glu Leu Gly Arg Gln Asp Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - Tyr Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 96                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 96                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Tyr Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Arg Val Ala Gl - #u Gln Leu Asn Gly Gln         #     60                                                                      - Lys Asp Thr Leu Glu Arg Glu Arg Ala Tyr Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 97                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 97                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Thr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Gln Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Glu Lys Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 98                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 98                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Thr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Gln Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Glu Lys Arg Ala Glu Va - #l Asp Arg Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 99                                                          <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 99                                                          - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Glu Arg Ser Phe Tyr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Arg Val Ala Gl - #u Gln Leu Asn Ser Gln         #     60                                                                      - Lys Asp Leu Leu Glu Ala Lys Arg Ala Asn Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 100                                                         <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 100                                                         - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Leu Le - #u Glu Arg Tyr Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Ala Glu Leu Gly Arg Pro Asp Ala Ly - #s Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Ser Arg Arg Thr Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 101                                                         <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 101                                                         - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Asn Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Ser Arg Arg Thr Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Phe Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 102                                                         <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 102                                                         - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asn Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 103                                                         <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 103                                                         - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Gln Arg Asp Ala Gl - #u Tyr Cys Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 104                                                         <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 104                                                         - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Th - #r Lys Lys Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Ly - #s Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 105                                                         <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 105                                                         - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Tyr Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Arg Val Ala Gl - #u Gln Leu Asn Gly Gln         #     60                                                                      - Lys Asp Thr Leu Glu Arg Glu Arg Ala Tyr Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 106                                                         <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 106                                                         - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Hi - #s Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe Tyr         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Ala Glu Leu Gly Arg Pro Glu Ala Ly - #s Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Lys Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Ile Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 107                                                         <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 107                                                         - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Ty - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Tyr Thr         #             30                                                              - Asn Gly Glu Glu Thr Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Leu Thr Glu Leu Gly Arg Gln Asp Ala Gl - #u Gln Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - Tyr Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 108                                                         <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 108                                                         - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Glu Ile Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Gly Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 109                                                         <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 109                                                         - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Thr Ser Glu Cys His         #                 15                                                          - Phe Ser Asn Gly Thr Glu Arg Val Arg Leu Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Phe Val Arg Phe Asp Ser As - #p Trp Gly Glu Phe Arg         #         45                                                                  - Ala Val Ala Glu Leu Gly Arg Pro Asp Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Ser Arg Arg Thr Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Ile Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 110                                                         <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 110                                                         - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Gly Glu Tyr Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Gln Arg Val Ala Gl - #u Tyr Cys Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Ala Arg Ala Ala Va - #l Asp Thr Tyr Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Gly Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 111                                                         <211> LENGTH: 94                                                              <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 111                                                         - Arg Glu Ile Gln Pro His Phe Leu Glu Tyr Se - #r Lys Ser Glu Cys His         #                 15                                                          - Phe Phe Asn Gly Thr Glu Arg Val Arg Phe Le - #u Asp Arg Tyr Phe His         #             30                                                              - Asn Gly Glu Glu Tyr Val Arg Phe Asp Ser As - #p Trp Asp Glu Phe Arg         #         45                                                                  - Ala Val Thr Glu Leu Gly Arg Pro Ser Ala Gl - #u Tyr Trp Asn Ser Gln         #     60                                                                      - Lys Asp Phe Leu Glu Arg Arg Arg Ala Glu Va - #l Asp Thr Val Cys Arg         # 80                                                                          - His Asn Tyr Gly Val Val Glu Ser Phe Thr Va - #l Gln Arg Arg                 #                 90                                                          - <210> SEQ ID NO 112                                                         <211> LENGTH: 4                                                               <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 112                                                         - Val Asp Thr Val                                                               1                                                                           - <210> SEQ ID NO 113                                                         <211> LENGTH: 4                                                               <212> TYPE: PRT                                                               <213> ORGANISM: BOVINE                                                        - <400> SEQUENCE: 113                                                         - Val Asp Arg Val                                                               1                                                                           - <210> SEQ ID NO 114                                                         <211> LENGTH: 18                                                              <212> TYPE: DNA                                                               <213> ORGANISM: Artificial Sequence                                           <220> FEATURE:                                                                #Sequence: PRIMERMATION: Description of Artificial                            - <400> SEQUENCE: 114                                                         #  18              gt                                                         - <210> SEQ ID NO 115                                                         <211> LENGTH: 18                                                              <212> TYPE: DNA                                                               <213> ORGANISM: Artificial Sequence                                           <220> FEATURE:                                                                #Sequence: PRIMERMATION: Description of Artificial                            - <400> SEQUENCE: 115                                                         #  18              cc                                                         __________________________________________________________________________

What is claimed is:
 1. A method for judging a resistance to the onset ofbovine leukemia caused by the bovine leukemia virus BLV, wherein abovine individual, in whic an amino acid defined by the amino acidnumber 78 of the β1 domain of the bovine MHC Class II DR β chain is Val,is judged to have resistance to the onset of the leukemia.
 2. The methodaccording to claim 1, the bovine individual, in which the amino acidspecified by the amino acid number 78 of the β1 domain of the bovine MHCClass II DRβ chain is Val in at least one of the alleles, is judged tohave resistance to the onset.
 3. The method according to claim 1 whereinthe bovine individual, in which the amino acid specified by the aminoacid number 78 of the β1 domain of the bovine MHC Class II DRβ chain isVal in both of the alleles, is judged to have high resistance to theonset.
 4. A method for judging a resistance to the onset of bovineleukemia caused by bovine leukemia virus BLV, which comprises the stepsof:(1) amplifying genome DNA isolated from a bovine individual by thepolymerase chain reaction to prepare a PCR product containing a DNAcoding for a part or full length of the β1 domain of the bovine MHCClass II DRβ chain, and (2) judging that the bovine individual, in whichan amino acid corresponding to the amino acid number 78 of the β1 domainof the bovine MHC Class II DRβ chain is Val in the amino acid sequenceencoded by the DNA contained in the PCR product, has a resistance to theonset of the leukemia.
 5. The method according to claim 4 whichcomprises a step of digesting the PCR product by using PstI.
 6. A primerset used for judgement of a possibility of onset of bovine leukemiacaused by bovine leukemia virus BLV or a resistance thereto, whichcomprises:(a) A primer:5'-TGTAAAACGACGGCCAGTCTCTCTCTGCAGCACATTTCCT-3'(SEQ ID NO: 1) and (b) Bprimer: 5'-CAGGAAACAGCTATGACCCGCCGCTGCACAGTGAAACTC-3'(SEQ ID NO: 2). 7.A primer set used for judgement of a possibility of onset of bovineleukemia caused by bovine leukemia virus BLV or a resistance thereto,which comprises:(a) A primer: 5'-GGAATTCCTCTCTCTGCAGCACATTTCCT-3'(SEQ IDNO: 3) and (b) B primer: 5'-AAGTCGACCGCTG(CACAGTGAAACTC-3'(SEQ ID NO:4).
 8. A primer set used for judgement of a possibility of onset ofbovine leukemia caused by bovine leukemia virus BLV or a resistancethereto. which comprises:(a) A primer which is selected from the groupconsisting of5'-GAGTGTCATTTCTTCAACGGGAC-3'(SEQ ID NO: 5)5'-GGAGAAGAGTTCGTGCGCTTCGA-3'(SEQ ID NO: 6), and5'-GGAATTCCTCTCTCTGCAGCACATTTCCT-3'(SEQ ID NO: 7), and (b) primer:5'-AAGTCGACCGCTGCACAGTGAAACTC-3'(SEQ ID NO: 8).